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    AddexBio Inc hob cells addexbio p0004010
    A) XTT cell viability assay of human osteoblasts <t>(HOB)</t> <t>cells</t> after treatment with TiO 2 NPs (a) and MPs (b) for a period of 24h. Data are represented as mean ± standard deviation. Statistical significance was observed among the different concentrations of TiO 2 NP, and MP administered mainly at lower concentrations when compared to non-treated cells. Significance was represented with *, **, *** and **** which represents a p -value <0.5, 0.01, 0.001 and 0.0001, respectively. B) Representative phase contrast microscopic images of the TiO 2 NPs and MPs treated HOB cells. The upper panel (a, b, c) represents the treatments with 5 μg/mL, 20 μg/mL, and 100 μg/mL TiO 2 NPs treatment and the lower panel (a(i), b(i), c(i) represents the TiO 2 MPs treatment. The morphological changes after treatment were compared with that of control HOB cells (d).
    Hob Cells Addexbio P0004010, supplied by AddexBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hob+cells+addexbio+p0004010/hob+cells+addexbio+p0004010/pmc11187976-42-0-5
    Average 90 stars, based on 1 article reviews
    hob cells addexbio p0004010 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study"

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study

    Journal: Biomaterial Investigations in Dentistry

    doi: 10.2340/biid.v11.40843

    A) XTT cell viability assay of human osteoblasts (HOB) cells after treatment with TiO 2 NPs (a) and MPs (b) for a period of 24h. Data are represented as mean ± standard deviation. Statistical significance was observed among the different concentrations of TiO 2 NP, and MP administered mainly at lower concentrations when compared to non-treated cells. Significance was represented with *, **, *** and **** which represents a p -value <0.5, 0.01, 0.001 and 0.0001, respectively. B) Representative phase contrast microscopic images of the TiO 2 NPs and MPs treated HOB cells. The upper panel (a, b, c) represents the treatments with 5 μg/mL, 20 μg/mL, and 100 μg/mL TiO 2 NPs treatment and the lower panel (a(i), b(i), c(i) represents the TiO 2 MPs treatment. The morphological changes after treatment were compared with that of control HOB cells (d).
    Figure Legend Snippet: A) XTT cell viability assay of human osteoblasts (HOB) cells after treatment with TiO 2 NPs (a) and MPs (b) for a period of 24h. Data are represented as mean ± standard deviation. Statistical significance was observed among the different concentrations of TiO 2 NP, and MP administered mainly at lower concentrations when compared to non-treated cells. Significance was represented with *, **, *** and **** which represents a p -value <0.5, 0.01, 0.001 and 0.0001, respectively. B) Representative phase contrast microscopic images of the TiO 2 NPs and MPs treated HOB cells. The upper panel (a, b, c) represents the treatments with 5 μg/mL, 20 μg/mL, and 100 μg/mL TiO 2 NPs treatment and the lower panel (a(i), b(i), c(i) represents the TiO 2 MPs treatment. The morphological changes after treatment were compared with that of control HOB cells (d).

    Techniques Used: Viability Assay, Standard Deviation, Control

    Representative confocal microscopic images showing the cytoskeletal organization of human osteoblast (HOB) cells after being treated with different concentrations and size of TiO 2 particles after 48h of incubation. Upper panel was HOBs treated with 5, 20 and 100 μg/mL (a, b, c, respectively) of TiO 2 NPs and lower panel was HOB treated with 5, 20 and 100 μg/mL (d, e, f, respectively) of TiO 2 MPs. There was no alteration in the cytoskeletal arrangement among the treated HOBs and their post-treatment morphology after 48 h was comparable to that of the non-treated control HOB cells (g). Texas red phalloidin was used for actin staining and DAPI (blue) for nuclei. The images were captured under 60X objective.
    Figure Legend Snippet: Representative confocal microscopic images showing the cytoskeletal organization of human osteoblast (HOB) cells after being treated with different concentrations and size of TiO 2 particles after 48h of incubation. Upper panel was HOBs treated with 5, 20 and 100 μg/mL (a, b, c, respectively) of TiO 2 NPs and lower panel was HOB treated with 5, 20 and 100 μg/mL (d, e, f, respectively) of TiO 2 MPs. There was no alteration in the cytoskeletal arrangement among the treated HOBs and their post-treatment morphology after 48 h was comparable to that of the non-treated control HOB cells (g). Texas red phalloidin was used for actin staining and DAPI (blue) for nuclei. The images were captured under 60X objective.

    Techniques Used: Incubation, Control, Staining

    Bar chart representing the percentage of Reactive Oxygen Species (ROS) generation (mean ± SD) of each human osteoblast (HOB) cell treated with titanium dioxide nanoparticles (TiO 2 NPs) and microparticles (MPs) with respect to control HOB (Cells). Statistical significance is represented as **** which signifies the p -values < 0.0001.
    Figure Legend Snippet: Bar chart representing the percentage of Reactive Oxygen Species (ROS) generation (mean ± SD) of each human osteoblast (HOB) cell treated with titanium dioxide nanoparticles (TiO 2 NPs) and microparticles (MPs) with respect to control HOB (Cells). Statistical significance is represented as **** which signifies the p -values < 0.0001.

    Techniques Used: Titanium Dioxide, Control

    Alkaline phosphatase (ALP) activity of human osteoblasts (HOBs) after 7 and 14 d of treatment with titanium dioxide nanoparticles (TiO 2 NPs) and microparticles (MPs) in normal media (NM) and osteogenic media (OM). Increase in ALP activity was observed at 14 d of culture conditions only. Statistically significant differences were observed in the ALP activity of TiO 2 treated HOBs in OM when compared with untreated cells grown in NM. Those cells treated with 100 μg/mL NPs in OM (100NPOM) demonstrated a high ALP activity at 14 d compared to TiO 2 MPs and untreated cells in NM and OM. Cells treated with 100 μg/mL MPs in OM (100MPOM) showed a lower ALP activity compared to those treated with NPs and untreated HOB cells cultured in OM. Significance was represented with * and **** which represents a p -value <0.5 and 0.0001, respectively.
    Figure Legend Snippet: Alkaline phosphatase (ALP) activity of human osteoblasts (HOBs) after 7 and 14 d of treatment with titanium dioxide nanoparticles (TiO 2 NPs) and microparticles (MPs) in normal media (NM) and osteogenic media (OM). Increase in ALP activity was observed at 14 d of culture conditions only. Statistically significant differences were observed in the ALP activity of TiO 2 treated HOBs in OM when compared with untreated cells grown in NM. Those cells treated with 100 μg/mL NPs in OM (100NPOM) demonstrated a high ALP activity at 14 d compared to TiO 2 MPs and untreated cells in NM and OM. Cells treated with 100 μg/mL MPs in OM (100MPOM) showed a lower ALP activity compared to those treated with NPs and untreated HOB cells cultured in OM. Significance was represented with * and **** which represents a p -value <0.5 and 0.0001, respectively.

    Techniques Used: Activity Assay, Titanium Dioxide, Cell Culture

    (A) Representative images of human osteoblast (HOB) cells stained with Alizarin Red S after 14 d of treatment with TiO 2 nanoparticles (TiO 2 NPs) (panel a) and TiO 2 microparticles (TiO 2 MP) (panel b) and untreated control HOB (Cells) (panel c) in normal media (NM) and osteogenic media (OM). As evident from the images, higher amount of calcium deposits was seen on untreated cells as well as in HOB treated with100 μg/mL of titanium dioxide nanoparticles in OM (100 NPOM). (B) represents the quantification of mineralized nodules formed in the wells after 7 d (a) and 14 d (b) of culture. The results are represented as mean ± standard deviation from three independent experiments done in triplicates. **** represents p -value < 0.0001.
    Figure Legend Snippet: (A) Representative images of human osteoblast (HOB) cells stained with Alizarin Red S after 14 d of treatment with TiO 2 nanoparticles (TiO 2 NPs) (panel a) and TiO 2 microparticles (TiO 2 MP) (panel b) and untreated control HOB (Cells) (panel c) in normal media (NM) and osteogenic media (OM). As evident from the images, higher amount of calcium deposits was seen on untreated cells as well as in HOB treated with100 μg/mL of titanium dioxide nanoparticles in OM (100 NPOM). (B) represents the quantification of mineralized nodules formed in the wells after 7 d (a) and 14 d (b) of culture. The results are represented as mean ± standard deviation from three independent experiments done in triplicates. **** represents p -value < 0.0001.

    Techniques Used: Staining, Control, Titanium Dioxide, Standard Deviation

    Related Articles

    Cell Culture:

    Article Title: In Vitro Cytotoxicity and Mineralization Potential of an Endodontic Bioceramic Material
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, California, United States) and maintained in DMEM/F-12 culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich).

    Article Title: Impact of exposure of human osteoblast cells to titanium dioxide particles in-vitro.
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, CA), and maintained in a DMEM/F-12 culture medium (Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich, USA).

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study
    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Viability Assay:

    Article Title: In Vitro Cytotoxicity and Mineralization Potential of an Endodontic Bioceramic Material
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, California, United States) and maintained in DMEM/F-12 culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich).

    Article Title: Impact of exposure of human osteoblast cells to titanium dioxide particles in-vitro.
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, CA), and maintained in a DMEM/F-12 culture medium (Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich, USA).

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study
    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Standard Deviation:

    Article Title: In Vitro Cytotoxicity and Mineralization Potential of an Endodontic Bioceramic Material
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, California, United States) and maintained in DMEM/F-12 culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich).

    Article Title: Impact of exposure of human osteoblast cells to titanium dioxide particles in-vitro.
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, CA), and maintained in a DMEM/F-12 culture medium (Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich, USA).

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study
    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Control:

    Article Title: In Vitro Cytotoxicity and Mineralization Potential of an Endodontic Bioceramic Material
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, California, United States) and maintained in DMEM/F-12 culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich).

    Article Title: Impact of exposure of human osteoblast cells to titanium dioxide particles in-vitro.
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, CA), and maintained in a DMEM/F-12 culture medium (Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich, USA).

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study
    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Incubation:

    Article Title: In Vitro Cytotoxicity and Mineralization Potential of an Endodontic Bioceramic Material
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, California, United States) and maintained in DMEM/F-12 culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich).

    Article Title: Impact of exposure of human osteoblast cells to titanium dioxide particles in-vitro.
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, CA), and maintained in a DMEM/F-12 culture medium (Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich, USA).

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study
    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Staining:

    Article Title: In Vitro Cytotoxicity and Mineralization Potential of an Endodontic Bioceramic Material
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, California, United States) and maintained in DMEM/F-12 culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich).

    Article Title: Impact of exposure of human osteoblast cells to titanium dioxide particles in-vitro.
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, CA), and maintained in a DMEM/F-12 culture medium (Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich, USA).

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study
    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Titanium Dioxide:

    Article Title: In Vitro Cytotoxicity and Mineralization Potential of an Endodontic Bioceramic Material
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, California, United States) and maintained in DMEM/F-12 culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich).

    Article Title: Impact of exposure of human osteoblast cells to titanium dioxide particles in-vitro.
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, CA), and maintained in a DMEM/F-12 culture medium (Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich, USA).

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study
    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Activity Assay:

    Article Title: In Vitro Cytotoxicity and Mineralization Potential of an Endodontic Bioceramic Material
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, California, United States) and maintained in DMEM/F-12 culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich).

    Article Title: Impact of exposure of human osteoblast cells to titanium dioxide particles in-vitro.
    Article Snippet: HOB cells were obtained from AddexBio (AddexBio P0004010, San Diego, CA), and maintained in a DMEM/F-12 culture medium (Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich, USA).

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study
    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .



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    AddexBio Inc hob cells addexbio p0004010
    A) XTT cell viability assay of human osteoblasts <t>(HOB)</t> <t>cells</t> after treatment with TiO 2 NPs (a) and MPs (b) for a period of 24h. Data are represented as mean ± standard deviation. Statistical significance was observed among the different concentrations of TiO 2 NP, and MP administered mainly at lower concentrations when compared to non-treated cells. Significance was represented with *, **, *** and **** which represents a p -value <0.5, 0.01, 0.001 and 0.0001, respectively. B) Representative phase contrast microscopic images of the TiO 2 NPs and MPs treated HOB cells. The upper panel (a, b, c) represents the treatments with 5 μg/mL, 20 μg/mL, and 100 μg/mL TiO 2 NPs treatment and the lower panel (a(i), b(i), c(i) represents the TiO 2 MPs treatment. The morphological changes after treatment were compared with that of control HOB cells (d).
    Hob Cells Addexbio P0004010, supplied by AddexBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hob+cells+addexbio+p0004010/hob+cells+addexbio+p0004010/pmc11187976-42-0-5
    Average 90 stars, based on 1 article reviews
    hob cells addexbio p0004010 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    A) XTT cell viability assay of human osteoblasts (HOB) cells after treatment with TiO 2 NPs (a) and MPs (b) for a period of 24h. Data are represented as mean ± standard deviation. Statistical significance was observed among the different concentrations of TiO 2 NP, and MP administered mainly at lower concentrations when compared to non-treated cells. Significance was represented with *, **, *** and **** which represents a p -value <0.5, 0.01, 0.001 and 0.0001, respectively. B) Representative phase contrast microscopic images of the TiO 2 NPs and MPs treated HOB cells. The upper panel (a, b, c) represents the treatments with 5 μg/mL, 20 μg/mL, and 100 μg/mL TiO 2 NPs treatment and the lower panel (a(i), b(i), c(i) represents the TiO 2 MPs treatment. The morphological changes after treatment were compared with that of control HOB cells (d).

    Journal: Biomaterial Investigations in Dentistry

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study

    doi: 10.2340/biid.v11.40843

    Figure Lengend Snippet: A) XTT cell viability assay of human osteoblasts (HOB) cells after treatment with TiO 2 NPs (a) and MPs (b) for a period of 24h. Data are represented as mean ± standard deviation. Statistical significance was observed among the different concentrations of TiO 2 NP, and MP administered mainly at lower concentrations when compared to non-treated cells. Significance was represented with *, **, *** and **** which represents a p -value <0.5, 0.01, 0.001 and 0.0001, respectively. B) Representative phase contrast microscopic images of the TiO 2 NPs and MPs treated HOB cells. The upper panel (a, b, c) represents the treatments with 5 μg/mL, 20 μg/mL, and 100 μg/mL TiO 2 NPs treatment and the lower panel (a(i), b(i), c(i) represents the TiO 2 MPs treatment. The morphological changes after treatment were compared with that of control HOB cells (d).

    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Techniques: Viability Assay, Standard Deviation, Control

    Representative confocal microscopic images showing the cytoskeletal organization of human osteoblast (HOB) cells after being treated with different concentrations and size of TiO 2 particles after 48h of incubation. Upper panel was HOBs treated with 5, 20 and 100 μg/mL (a, b, c, respectively) of TiO 2 NPs and lower panel was HOB treated with 5, 20 and 100 μg/mL (d, e, f, respectively) of TiO 2 MPs. There was no alteration in the cytoskeletal arrangement among the treated HOBs and their post-treatment morphology after 48 h was comparable to that of the non-treated control HOB cells (g). Texas red phalloidin was used for actin staining and DAPI (blue) for nuclei. The images were captured under 60X objective.

    Journal: Biomaterial Investigations in Dentistry

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study

    doi: 10.2340/biid.v11.40843

    Figure Lengend Snippet: Representative confocal microscopic images showing the cytoskeletal organization of human osteoblast (HOB) cells after being treated with different concentrations and size of TiO 2 particles after 48h of incubation. Upper panel was HOBs treated with 5, 20 and 100 μg/mL (a, b, c, respectively) of TiO 2 NPs and lower panel was HOB treated with 5, 20 and 100 μg/mL (d, e, f, respectively) of TiO 2 MPs. There was no alteration in the cytoskeletal arrangement among the treated HOBs and their post-treatment morphology after 48 h was comparable to that of the non-treated control HOB cells (g). Texas red phalloidin was used for actin staining and DAPI (blue) for nuclei. The images were captured under 60X objective.

    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Techniques: Incubation, Control, Staining

    Bar chart representing the percentage of Reactive Oxygen Species (ROS) generation (mean ± SD) of each human osteoblast (HOB) cell treated with titanium dioxide nanoparticles (TiO 2 NPs) and microparticles (MPs) with respect to control HOB (Cells). Statistical significance is represented as **** which signifies the p -values < 0.0001.

    Journal: Biomaterial Investigations in Dentistry

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study

    doi: 10.2340/biid.v11.40843

    Figure Lengend Snippet: Bar chart representing the percentage of Reactive Oxygen Species (ROS) generation (mean ± SD) of each human osteoblast (HOB) cell treated with titanium dioxide nanoparticles (TiO 2 NPs) and microparticles (MPs) with respect to control HOB (Cells). Statistical significance is represented as **** which signifies the p -values < 0.0001.

    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Techniques: Titanium Dioxide, Control

    Alkaline phosphatase (ALP) activity of human osteoblasts (HOBs) after 7 and 14 d of treatment with titanium dioxide nanoparticles (TiO 2 NPs) and microparticles (MPs) in normal media (NM) and osteogenic media (OM). Increase in ALP activity was observed at 14 d of culture conditions only. Statistically significant differences were observed in the ALP activity of TiO 2 treated HOBs in OM when compared with untreated cells grown in NM. Those cells treated with 100 μg/mL NPs in OM (100NPOM) demonstrated a high ALP activity at 14 d compared to TiO 2 MPs and untreated cells in NM and OM. Cells treated with 100 μg/mL MPs in OM (100MPOM) showed a lower ALP activity compared to those treated with NPs and untreated HOB cells cultured in OM. Significance was represented with * and **** which represents a p -value <0.5 and 0.0001, respectively.

    Journal: Biomaterial Investigations in Dentistry

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study

    doi: 10.2340/biid.v11.40843

    Figure Lengend Snippet: Alkaline phosphatase (ALP) activity of human osteoblasts (HOBs) after 7 and 14 d of treatment with titanium dioxide nanoparticles (TiO 2 NPs) and microparticles (MPs) in normal media (NM) and osteogenic media (OM). Increase in ALP activity was observed at 14 d of culture conditions only. Statistically significant differences were observed in the ALP activity of TiO 2 treated HOBs in OM when compared with untreated cells grown in NM. Those cells treated with 100 μg/mL NPs in OM (100NPOM) demonstrated a high ALP activity at 14 d compared to TiO 2 MPs and untreated cells in NM and OM. Cells treated with 100 μg/mL MPs in OM (100MPOM) showed a lower ALP activity compared to those treated with NPs and untreated HOB cells cultured in OM. Significance was represented with * and **** which represents a p -value <0.5 and 0.0001, respectively.

    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Techniques: Activity Assay, Titanium Dioxide, Cell Culture

    (A) Representative images of human osteoblast (HOB) cells stained with Alizarin Red S after 14 d of treatment with TiO 2 nanoparticles (TiO 2 NPs) (panel a) and TiO 2 microparticles (TiO 2 MP) (panel b) and untreated control HOB (Cells) (panel c) in normal media (NM) and osteogenic media (OM). As evident from the images, higher amount of calcium deposits was seen on untreated cells as well as in HOB treated with100 μg/mL of titanium dioxide nanoparticles in OM (100 NPOM). (B) represents the quantification of mineralized nodules formed in the wells after 7 d (a) and 14 d (b) of culture. The results are represented as mean ± standard deviation from three independent experiments done in triplicates. **** represents p -value < 0.0001.

    Journal: Biomaterial Investigations in Dentistry

    Article Title: Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study

    doi: 10.2340/biid.v11.40843

    Figure Lengend Snippet: (A) Representative images of human osteoblast (HOB) cells stained with Alizarin Red S after 14 d of treatment with TiO 2 nanoparticles (TiO 2 NPs) (panel a) and TiO 2 microparticles (TiO 2 MP) (panel b) and untreated control HOB (Cells) (panel c) in normal media (NM) and osteogenic media (OM). As evident from the images, higher amount of calcium deposits was seen on untreated cells as well as in HOB treated with100 μg/mL of titanium dioxide nanoparticles in OM (100 NPOM). (B) represents the quantification of mineralized nodules formed in the wells after 7 d (a) and 14 d (b) of culture. The results are represented as mean ± standard deviation from three independent experiments done in triplicates. **** represents p -value < 0.0001.

    Article Snippet: HOB cells were procured from AddexBio (AddexBio P0004010, San Diego, CA, USA) and were maintained in DMEM/F-12 cell culture medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a CO 2 incubator with 5% CO 2 .

    Techniques: Staining, Control, Titanium Dioxide, Standard Deviation